polyclonal anti-human parc  (PeproTech)

Journal: PLoS ONE

Article Title: Gene Expression Profiling of Human Decidual Macrophages: Evidence for Immunosuppressive Phenotype

doi: 10.1371/journal.pone.0002078

Figure Lengend Snippet: Selection of genes differentially expressed in decidual compared to blood CD14 + cells in early pregnancy.

Article Snippet: The protein level of CCL18 was determined by an in-house double-antibody sandwich ELISA using a monoclonal anti-human CCL18 (0.5 μg/mL;clone 64507; R&D Systems, Abingdon, UK) and a polyclonal biotinylated anti-human CCL18 Antibody (200 ng/ml; R&D Systems).

Techniques: Selection, Membrane, Binding Assay, Clinical Proteomics

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Unique regulation of CCL18 production by maturing dendritic cells.

doi: 10.4049/jimmunol.170.7.3843

Figure Lengend Snippet: FIGURE 1. Opposite kinetics of CCL18 and CCL20 production in ma- turing DC. DC (106/ml) were cultured in the absence (control) or the pres- ence of LPS (100 ng/ml). Supernatants were collected at different time points and were tested for chemokine production by specific ELISA. Re- sults are expressed as the mean (SD) of 4–15 independent experiments. , p 0.05 vs control.

Article Snippet: An in-house sandwich ELISA for CCL18 was developed by coating plates with polyclonal anti-human CCL18 Ab (R&D Systems, Abingdon, U.K.).

Techniques: Cell Culture, Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Unique regulation of CCL18 production by maturing dendritic cells.

doi: 10.4049/jimmunol.170.7.3843

Figure Lengend Snippet: FIGURE 2. Different regulation of CCL18 and CCL20 production by maturing DC. DC (106/ml) were incubated in the absence () or the pres- ence of 100 ng/ml LPS, 20 ng/ml TNF, or CD40L (4:1 ratio of DC to CD40L transfectants) for 48 h. Supernatants were evaluated for the pres- ence of chemokines by ELISA. The top panels show chemokine levels. Results are the average determinations (SD) of eight independent exper- iments. The lower panels report Northern blot analysis of total RNA (10 g/lane) purified from DC in one experiment representative of four. , p 0.05; , p 0.01 (vs control ()).

Article Snippet: An in-house sandwich ELISA for CCL18 was developed by coating plates with polyclonal anti-human CCL18 Ab (R&D Systems, Abingdon, U.K.).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Northern Blot, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Unique regulation of CCL18 production by maturing dendritic cells.

doi: 10.4049/jimmunol.170.7.3843

Figure Lengend Snippet: FIGURE 3. Regulation of CCL18 production by IFN- and IL-10. DC (106/ml) were cultured with LPS (100 ng/ml), IFN- (500 U/ml), or both LPS and IFN- (A) and with IL-10 (20 ng/ml) or IL-10 and LPS (B). Supernatants were collected at different time points and were tested for CCL18 production by ELISA. Protein levels are expressed as the mean (SD) of three independent experiments. , p 0.01; #, p 0.05 (vs control ()).

Article Snippet: An in-house sandwich ELISA for CCL18 was developed by coating plates with polyclonal anti-human CCL18 Ab (R&D Systems, Abingdon, U.K.).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Unique regulation of CCL18 production by maturing dendritic cells.

doi: 10.4049/jimmunol.170.7.3843

Figure Lengend Snippet: FIGURE 4. Induction of CCL18 in immature DC by VitD3. A, DC (106/ ml) were incubated in medium alone (control) or in the presence of VitD3 (107 M) for 4 h and then stimulated with LPS (100 ng/ml) for 48 h. Supernatants were tested by ELISA. Results are the average determinations (SD) of three to seven independent experiments. The average (100%) CCL18 (by immature DC) and CCL20 (by mature DC) productions were 366 55 and 1.8 0.6 ng/106 DC, respectively. B, Total RNA was isolated from immature DC and mature DC that had been stimulated with LPS or CD40L for 48 h. The expression of VitD3 receptor (VD3R) mRNA was analyzed by RT-PCR as described in Materials and Methods. , p 0.01 vs control (); #, p 0.05 vs LPS.

Article Snippet: An in-house sandwich ELISA for CCL18 was developed by coating plates with polyclonal anti-human CCL18 Ab (R&D Systems, Abingdon, U.K.).

Techniques: Incubation, Control, Enzyme-linked Immunosorbent Assay, Isolation, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Unique regulation of CCL18 production by maturing dendritic cells.

doi: 10.4049/jimmunol.170.7.3843

Figure Lengend Snippet: FIGURE 6. Migration of immature DC in response to CCL18. Imma- ture DC (iDC) were assayed for their migratory capacity toward recombi- nant (R&D Systems or PeproTech) and purified natural CCL18 (39). The migration assay was performed as indicated in Materials and Methods. Results are expressed as the mean (SD) of three replicates of a single experiment and are representative of at least three independent donors. Basal migration, against medium (12 2 cells), was subtracted from the data. The dashed line indicates the number of migrated cells in response to 100 ng/ml CCL3. , p 0.05; , p 0.01 vs medium (no chemokine).

Article Snippet: An in-house sandwich ELISA for CCL18 was developed by coating plates with polyclonal anti-human CCL18 Ab (R&D Systems, Abingdon, U.K.).

Techniques: Migration

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Unique regulation of CCL18 production by maturing dendritic cells.

doi: 10.4049/jimmunol.170.7.3843

Figure Lengend Snippet: FIGURE 5. CCL18 and CCL 20 production is differentially regulated during alternative pathways of DC maturation. DC (106/ml) were incubated in the presence of Dex (105 M) or PGE2 (105M) for 4 h and then stimulated with LPS (100 ng/ml), CD40L, or medium alone (control) for 48 h. Supernatants were tested by ELISA. Results are the average deter- minations (SD) of eight independent experiments. Protein production in the absence of Dex or PGE2 was considered as 100% of chemokine pro- duction and corresponded to 380 6.4 ng/ml CCL18 in DC control (A), and 0.25 0.02 and 0.68 0.02 ng/ml IL-12 in LPS-stimulated DC and CD40L-stimulated DC, respectively (B). , p 0.05 vs respective no ad- dition (). No production of IL-12 was observed in DC control superna- tants (not shown).

Article Snippet: An in-house sandwich ELISA for CCL18 was developed by coating plates with polyclonal anti-human CCL18 Ab (R&D Systems, Abingdon, U.K.).

Techniques: Incubation, Control, Enzyme-linked Immunosorbent Assay